Catalog No: OKEH02495
Price: $675.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFLAR ELISA Kit (Human) (OKEH02495) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFLAR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFLAR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFLAR captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
CFLAR Microplate96 Wells (12 x 8 Well strips)
CFLAR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CFLAR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity18 pg/mL
SpecificityNatural and recombinant Human CASP8 and FADD-like apoptosis regulator
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFLAR
Gene Full NameCASP8 and FADD like apoptosis regulator
Alias SymbolsCASH;CASP8 and FADD-like apoptosis regulator;CASP8AP1;caspase homolog;caspase-eight-related protein;caspase-like apoptosis regulatory protein;caspase-related inducer of apoptosis;Casper;cellular FLICE-like inhibitory protein;cFLIP;c-FLIP;c-FLIPL;c-FLIPR;c-FLIPS;CLARP;FADD-like antiapoptotic molecule 1;FLAME;FLAME1;FLAME-1;FLIP;I-FLICE;inhibitor of FLICE;MACH-related inducer of toxicity;MRIT;testis secretory sperm-binding protein Li 233m;Usurpin;usurpin beta.
NCBI Gene Id8837
Protein NameO15519
Description of TargetCASP8 and FADD-like apoptosis regulator
Uniprot ID
Protein Accession #NP_001120655.1
Nucleotide Accession #NM_001127183.2
Protein Size (# AA)480
Molecular Weight55 kDa
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