CD74 Antibody : Biotin (OASE00026-Biotin)

Catalog No: OASE00026-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: PIN.1
• Isotype: IgG
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IP, IC, FC, FACS
• Additional Information: Background Info: Detects~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS PAGE immunoblots.
  Scientific Background: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment.
  Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been
  reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
  Certificate of Analysis: 1ug/mL was sufficient for detection of in 20ug of PALA cell lysates by colorimetric immunoblot analysis using goat anti-mouse IgG: AP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Human CD74 invariant chain synthetic peptide
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1 ug/mL was sufficient for detection using colorimetric western blot analysis
• Storage Buffer: PBS pH7.2, 50% glycerol
• Reference: 1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.
  2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9.
  3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816.
  4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11.
  5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165.
  6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.

Target Info

• Gene Symbol: CD74
• Gene Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Alias Symbols: II, p33, DHLAG, HLADG, Ia-GAMMA
• NCBI Gene Id: 972
• Uniprot ID: P04233
• Protein Accession #: NP_001020329.1