CD74 Antibody (OAEE01081)

Catalog No: OAEE01081

Size:100UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: M5/114
• Isotype: IgG2b
• Host: Rat
• Conjugation: FITC
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Activated C57BL/6 mouse spleen cells
• Purification: Purified by size-exclusion chromatography
• Concentration: 0.5 mg/ml
• Specificity: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• Formulation: FITC
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8(2):143-55.
  Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec;16(12):3661-8.
  Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug;19(8):977-92.
  Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1;174(3):1205-12.
  Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1;155(1):53-63.
  De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct;108 ( Pt 10):3219-31.
  Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 26;72(4):635-48.
  Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127(6):2488-95.
  

Target Info

• Gene Symbol: CD74
• Gene Full Name: HLA class II histocompatibility antigen gamma chain
• Alias Symbols: II, p33, DHLAG, HLADG, Ia-GAMMA
• NCBI Gene Id: 972
• Protein Name: HLA class II histocompatibility antigen gamma chain
• Uniprot ID: P04233
• Protein Accession #: NP_001020329.1; NP_001020330.1; NP_004346.1
• Nucleotide Accession #: NM_001025158.2; NM_001025159.2; NM_004355.3