CD70/TNFSF7/cd27L ELISA Kit (Mouse) (OKBB00598)

Catalog No: OKBB00598

Size:96 Wells

Mouse CD70/TNFSF7/cd27L ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been precoated onto 96-well plates. Standards(NS0, S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD70 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	94	511	1032	148	525	1385
  SD	3.57	26.06	44.38	6.66	32.55	77.56
  CV%	3.8	5.1	4.3	4.5	6.2	5.6

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CD70 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CD70 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CD70 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CD70
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: S45-P195
• Description: For quantitative detection of mouse CD70 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CD70
• Gene Full Name: CD70 molecule
• Alias Symbols: CD 27L, CD 70, CD27 L, CD27 LG, CD27 ligand, CD27-L, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, CD70_HUMAN, Ki 24 antigen, Ki24 antigen, Surface antigen CD70, TNFSF 7, TNFSF7, Tumor necrosis factor (ligand) superfamily, member 7, Tumor necrosis factor ligand superfamily member
• NCBI Gene Id: 21948
• Protein Name: CD70 antigen
• Description of Target: CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
• Uniprot ID: O55237
• Protein Accession #: NP_035747.1
• Nucleotide Accession #: NM_011617.2