CD163 Antibody (OASA03871)

Catalog No: OASA03871 (Formerly GWB-Q01270)

Size:250UG

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: ED2
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
  Histology Positive Control Tissue: Liver
  Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 0.5 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
• Reconstitution and Storage: -20°C
• Immunogen: Rat Spleen cell homogenate.
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Rat
• Concentration: 0.5 mg/ml
• Specificity: CD163
• Application Info: Flow Cytometry:    1/10 - 1/100
  Immunohistology - Frozen:    1/50 - 1/100
  (1) Special conditions apply. - See [*].
• Application Data: Application #1: Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC
  Application #2: Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE
• Protocol Information: Citation: 1: Meacock SC, Brandon DR, Billingham ME. Arthritis in Lewis rats induced by the non-immunogenic adjuvant CP20961: an immunohistochemical analysis of the developing disease. Ann Rheum Dis. 1994 Oct;53(10):653-8. PubMed PMID: 7979577;PubMed Central PMCID: PMC1005431.
  Species: Rat
  Experiment Name: Immunohistoperoxidase technique
  Experiment Background: The role of lymphocytes and macrophages in developing adjuvant arthritis induced by an injection of CP20961 in inbred Lewis rats was studied over a 32 day period using a novel biotinavidin immunoperoxidase histochemical technique.
  Experimental Steps: 1. Fresh frozen sections of hind paws and spleens, as well as lymph nodes draining the site of the injected adjuvant were immunostained using a panel of monoclonal antibodies specific for subsets of lymphocytes and macrophages and for MHC Class II antigen.2. Cryostat sections (4 um) of spleen andlymph nodes were mounted on poly-l-lysinecoatedmultispot slides, allowed to dry for onehour and fixed in acetone for five minutes at40. The sections were then stained accordingto the immunohistoperoxidase technique. Control sections werestained with H&E.
  Other Reagents Used: CP20961, Tetraaminobiphenyl hydrochloride
  Number Of Protocols: 1
• Reference: 1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 54:589-599.
  2. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation. Transplant. Proc. 3: 3166-3170.
  3. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2. Immunobiol. 182: 88-99.
  4. Dijkstra, C.D. and Damoiseaux, J.G.M.C. (1993) Macrophage heterogeneity established by immunocytochemistry. Prog. Histochem. Cytochem. 27: 1-65.
  5. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators. PhD Thesis. Vrije University, Amsterdam.
  6. Whiteland, J.L., Nicholls, S.M., Shimeld, C., Easty, D.L., Williams, N.A. and Hill, T.J. (1995) Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43(3): 313-320.
  7. Deng, X. et al. (2005) Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats. Am. J. Pathol. 166: 93-106.
  8. Piquet-Pellorce, I. et al. (2005) Identification of the leukemia inhibitory factor cell targets within the rat testis. Biol Reprod. 72: 602-11
  

Target Info

• Gene Symbol: CD163
• Alias Symbols: PTHSL2, SCZD17, Hs.22998
• NCBI Gene Id: 9378
• Description of Target: MOUSE ANTI RAT CD163
  
• Uniprot ID: P58400
• Protein Size (# AA): 1116