CD163 Antibody (OASA03870)

Catalog No: OASA03870 (Formerly GWB-Q01269)

Size:100UG

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: ED2
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
  Histology Positive Control Tissue: Liver
  Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 0.5 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
• Reconstitution and Storage: -20°C
• Immunogen: Rat Spleen cell homogenate.
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Rat
• Concentration: 0.5 mg/ml
• Specificity: CD163
• Application Info: Flow Cytometry:    1/10 - 1/100
  Immunohistology - Frozen:    1/50 - 1/100
  (1) Special conditions apply. - See [*].
• Application Data: Application #1: Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC
  Application #2: Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE
• Protocol Information: Citation: 1: Vlassara H, Fuh H, Makita Z, Krungkrai S, Cerami A, Bucala R. Exogenous advanced glycosylation end products induce complex vascular dysfunction in normal animals: a model for diabetic and aging complications. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12043-7. PubMed PMID: 1465438; PubMed Central PMCID: PMC50694.
  Species: Rat
  Experiment Name: Immunostaining of tissues
  Experiment Background: HELEN et al. perfomed Immunostaining of tissues to analysis the Increased mononuclear cell migratory activity in tissues from normal rats treated with exogenous AGE-RSA.
  Experimental Steps: 1. After the i.v. injection of either unmodified RSA , AGE-RSA , or AGE-RSA plus aminoguanidine for 2 week, liver and kidney with 10% formaldehyde by standard procedures, paraffin-embedded tissue sections from all animal groups (n = 5 per group) were processed in triplicate for routine histological examination after staining with Harris' alum hematoxylin stain.2. The presence of monocytes / macrophages in tissues was determined by using the, macrophage-specific monoclonal antibody ED2 as primary antibody and biotinylated goat anti-mouse IgG [F(ab')2] (Cappel Research Products) as secondary antibody in 7 to 10-um sections of frozen tissues.
  Other Reagents Used: Acetone,chloroform
  Number Of Protocols: 1
• Reference: 1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 54:589-599.
  2. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation. Transplant. Proc. 3: 3166-3170.
  3. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2. Immunobiol. 182: 88-99.
  4. Dijkstra, C.D. and Damoiseaux, J.G.M.C. (1993) Macrophage heterogeneity established by immunocytochemistry. Prog. Histochem. Cytochem. 27: 1-65.
  5. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators. PhD Thesis. Vrije University, Amsterdam.
  6. Whiteland, J.L., Nicholls, S.M., Shimeld, C., Easty, D.L., Williams, N.A. and Hill, T.J. (1995) Immunohistochemical Detection of T-Cell Subsets and other Leucocytes in Paraffin-embedded Rat and Mouse Tissues with Monoclonal Antibodies. J. Histochem. Cytochem. 43(3): 313-320.
  7. Deng, X. et al. (2005) Chronic alcohol consumption accelerates fibrosis in response to cerulein-induced pancreatitis in rats. Am. J. Pathol. 166: 93-106.
  8. Piquet-Pellorce, I. et al. (2005) Identification of the leukemia inhibitory factor cell targets within the rat testis. Biol Reprod. 72: 602-11
  

Target Info

• Gene Symbol: CD163
• Alias Symbols: M130, MM130, SCARI1
• NCBI Gene Id: 9332
• Description of Target: MOUSE ANTI RAT CD163
  
• Uniprot ID: Q86VB7
• Protein Size (# AA): 1116