CCL5 ELISA Kit (Canine) : 96 Wells (OKCA00238)

Catalog No: OKCA00238

Size:96 Wells

Canine CCL5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canine
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	105%	97-110%
  Serum (n=4)	1:2	101%	95-106%
  Serum (n=4)	1:4	96%	87-101%
  Serum (n=4)	1:8	102%	97-107%

• ELISA Kit Principle: Aviva Systems Biology CCL5 ELISA Kit (Canine) (OKCA00238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Canine CCL5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Canine CCL5 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Canine CCL5 captured in well.
• ELISA Kit Range: 0.156 ng/mL - 10 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	104%	96-108%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	99%	91-104%

• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/ml)	1.234	1.208	1.253	1.225	1.227	1.202
  SD	0.026	0.022	0.028	0.046	0.049	0.051
  CV%	2.377	2.035	2.539	4.224	4.495	4.731

• ELISA Kit Component:
  • Anti-CCL5 Microplate: 96 Well (12 x 8 Well Strips)
  • CCL5 Standard (Lyophilized): 2 x 10 ng
  • 100X CCL5 Detection Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.081 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of dog CCL5. No significant cross-reactivity or interference between dog CCL5 and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CCL5
• Gene Full Name: chemokine (C-C motif) ligand 5
• Alias Symbols: RANTES
• NCBI Gene Id: 403522
• Protein Name: C-C motif chemokine 5
• Description of Target: This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
• Uniprot ID: Q8HYS0
• Protein Accession #: NP_001003010
• Nucleotide Accession #: NM_001003010
• Protein Size (# AA): 91
• Molecular Weight: 10 kDa