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Catalog No: OKBB00243
Size:96 Tests
Price: $583.00
SKU
OKBB00243
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes has been precoated onto 96-well plates. Standards(E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Rantes amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)147293501110339658
St. Dev.5.8815.5331.18.1424.4156.6
%CV45.306.207.47.208.60
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human Rantes standard10 ng/tube x 2
Anti-human Rantes Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human Rantes antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 15.6pg/ml-1000pg/ml
::Principle: Aviva’s human Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes has been precoated onto 96-well plates. Standards (E.coli, S24-S91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Rantes amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine
Sensitivity<3 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. Liu, H.; Chao, D.; Nakayama, E. E.; Taguchi, H.; Goto, M.; Xin, X.; Takamatsu, J.; Saito, H.; Ishikawa, Y.; Akaza, T.;
Juji, T.; Takebe, Y.; and 9 others. Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression.
Proc. Nat. Acad. Sci. 96: 4581-4585, 1999.
2. Schall, T. J.; Jongstra, J.; Dyer, B. J.; Jorgensen, J.; Clayberger, C.; Davis, M. M.; Krensky, A. M. A human T
cell-specific molecule is a member of a new gene family. J. Immun. 141: 1018-1025, 1988.
SpecificityNatural and recombinant human Rantes
ImmunogenExpression system for standard: E.coli; Immunogen sequence: S24-S91
DescriptionFor quantitative detection of human Rantes in cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine.
Gene SymbolCCL5
Gene Full Namechemokine (C-C motif) ligand 5
Alias SymbolsBeta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES(4-68), Regulated upon activation normally T expressed and presumably secreted, SCYA 5, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine A5 (RANTES), Small inducible cytokine subfamily A (Cys Cys) member 5, Small-inducible cytokine A5, T cell specific protein p288, T cell specific protein RANTES, T cell specific RANTES protein, T cell-specific protein P228, T-cell-specific protein RANTES, TCP 228, TCP228
NCBI Gene Id6352
Protein NameC-C motif chemokine 5
Description of TargetRantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2
Uniprot IDP13501
Protein Accession #NP_002976.2
Nucleotide Accession #NM_002985.2
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