CASP4 Recombinant Protein (Human) (OPED00018)

Catalog No: OPED00018

Size:5000U

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid.In 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 1mM EDTA, 10% glycerol, 10mM DTT
• Reconstitution and Storage: Store at -80C
  Ships with Dry Ice
• Concentration: 100 U/μL
• Purity: >= 90% (SDS-PAGE)
• Biological Activity: One U=1 pmol/min at 30C using Ac-LEHD-pNA (200 mM) as substrate.
• Source: E. Coli
• Reference: A whole cell assay to measure caspase-6 activity by detecting cleavage of lamin A/C: R. Mintzer, et al.; PLoS One 7, e30376 (2012), Abstract; Full Text
  Water-soluble carbon nanotube-enzyme conjugates as functional biocatalytic formulations: P. Asuri, et al.; Biotechnol. Bioeng. 95, 804 (2006), Abstract;
  Cleavage of p53-vimentin complex enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis of rheumatoid arthritis synovial fibroblasts: X. Yang, et al.; Am. J. Pathol. 167, 705 (2005), Abstract;
  KIPase activity is a novel caspase-like activity associated with cell proliferation: C. Medina-Palazon, et al.; Eur. J. Biochem. 271, 2716 (2004), Abstract; Full Text
  Printing chemical libraries on microarrays for fluid phase nanoliter reactions: D.N. Gosalia, et al.; Proc. Natl. Acad. Sci. U. S. A. 100, 8721 (2003), Abstract; Full Text

Target Info

• Gene Symbol: CASP4
• Gene Full Name: caspase 4
• Alias Symbols: apoptotic cysteine protease Mih1/TX;CASP-4;caspase 4, apoptosis-related cysteine peptidase;caspase 4, apoptosis-related cysteine protease;caspase-4;caspase-4 isoform alpha;ICE and Ced-3 homolog 2;ICE(rel)II;ICE(rel)-II;ICEREL-II;ICH-2;Mih1;Mih1/TX;protease ICH-2;protease TX;TX.
• NCBI Gene Id: 837
• Protein Name: Caspase-4
• Description of Target: Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).
• Uniprot ID: P49662
• Protein Size (# AA): Recombinant
• Molecular Weight: 19 kDa, 10 kDa