BTK Antibody (OAPB00259)

Catalog No: OAPB00259 (Formerly GWB-933E7F)

Size:100UG

Western blot analysis of BTK in U937 cell lysate with BTK antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.

Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 ug/ml.

Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 ug/mL.

Immunocytochemistry of BTK in Daudi cells with BTK antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Chicken|Human|Mouse
• Product Format: Liquid. BTK Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: U937 Cell Lysate (OCPB00167), Daudi Cell Lysate (OCPB00159)
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: BTK antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK. The immunogen is located within amino acids 20 - 70 of BTK.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BTK (OAPB00259) antibody is Catalog # OEPB02352
• Application Info: BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL and immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 10 ug/mL.
• Reference: Vetrie D, Vorechovsky I, Sideras P, et al. The gene involved in X-linked agammaglobulinaemia is a member of the src family of tyrosine kinases. Nature 1993; 361:226-33.;Kawakami Y, Kitaura J, Hata D, et al. Functions of Bruton's tyrosine kinase in mast and B cells. J. Leukoc. Biol. 1999; 65(3):286-90.;Jefferies CA, Doyla S, Brunner C, et al. Bruton’s tyrosine kinase is a Toll/Interleukin-1 receptor domain-binding protein that participates in nuclear factor kB activation by Toll-like receptor 4. J. Biol. Chem. 2003; 278:26258-26264.;Akira S and Takeda K. Toll-like receptor Signalling. Nat. Rev. Immunol. 2004; 4:499-511.

Target Info

• Gene Symbol: BTK
• Gene Full Name: Bruton tyrosine kinase
• Alias Symbols: agammaglobulinaemia tyrosine kinase;Agammaglobulinemia tyrosine kinase;AGMX1;AT;ATK;B-cell progenitor kinase;BPK;Bruton agammaglobulinemia tyrosine kinase;Bruton tyrosine kinase;Bruton's tyrosine kinase;dominant-negative kinase-deficient Brutons tyrosine kinase;IGHD3;IMD1;PSCTK1;truncated Bruton agammaglobulinemia tyrosine kinase;tyrosine-protein kinase BTK;XLA.
• NCBI Gene Id: 695
• Protein Name: Tyrosine-protein kinase BTK
• Description of Target: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
• Uniprot ID: Q06187
• Protein Accession #: NP_000052.1; NP_001274273.1; NP_001274274.1
• Nucleotide Accession #: NM_000061.2; NM_001287344.1; NM_001287345.1
• Molecular Weight: Predicted: 76 kDa Observed: 79 kDa