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Catalog No: OAAF00035 (Formerly GWB-ASB041)
Size:100 ug
Price: $344.00
SKU
OAAF00035
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for BTK Antibody (Phospho-Tyr223) (OAAF00035)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Western blot
Additional InformationModification Sites: H: Y223 M: Y223 R: Y224
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223.
PurificationThe antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Concentration1 mg/ml
SpecificityBTK (Phospho-Tyr223) Antibody detects endogenous levels of BTK only when phosphorylated at Tyr223.
Application InfoWB: 1:500~1:1000
ELISA: 1:40000
The optimal working dilution should be determined by the end user.
Gene SymbolBTK
Gene Full NameBruton tyrosine kinase
Alias Symbolsagammaglobulinaemia tyrosine kinase;Agammaglobulinemia tyrosine kinase;AGMX1;AT;ATK;B-cell progenitor kinase;BPK;Bruton agammaglobulinemia tyrosine kinase;Bruton tyrosine kinase;Bruton's tyrosine kinase;dominant-negative kinase-deficient Brutons tyrosine kinase;IGHD3;IMD1;PSCTK1;truncated Bruton agammaglobulinemia tyrosine kinase;tyrosine-protein kinase BTK;XLA.
NCBI Gene Id695
Protein NameTyrosine-protein kinase BTK
Description of TargetNon-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Uniprot IDQ06187
Protein Accession #NP_000052.1
Molecular Weight76 kDa
  1. What is the species homology for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "BTK Antibody (Phospho-Tyr223) (OAAF00035)" provided in?

    This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    This target may also be called "agammaglobulinaemia tyrosine kinase;Agammaglobulinemia tyrosine kinase;AGMX1;AT;ATK;B-cell progenitor kinase;BPK;Bruton agammaglobulinemia tyrosine kinase;Bruton tyrosine kinase;Bruton's tyrosine kinase;dominant-negative kinase-deficient Brutons tyrosine kinase;IGHD3;IMD1;PSCTK1;truncated Bruton agammaglobulinemia tyrosine kinase;tyrosine-protein kinase BTK;XLA." in publications.

  5. What is the shipping cost for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "76 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "BTK Antibody (Phospho-Tyr223) (OAAF00035)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "BTK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "BTK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "BTK"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "BTK"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "BTK"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "BTK"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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