BMP4 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03333)

Catalog No: OKCD03333

Size:96WELLS

Mouse BMP4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-103%	80-101%	78-95%	95-103%
  EDTA Plasma (n=5)	89-99%	84-91%	94-102%	82-93%
  Heparin Plasma (n=5)	89-99%	95-105%	89-103%	93-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-103	101
  EDTA Plasma (n=5)	79-90	84
  Heparin Plasma (n=5)	81-101	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Bmp4 Microplate: 96 Wells (12 x 8 Well strips)
  • Bmp4 Lyophilized Standard: 2
  • 100X Bmp4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bmp4
• Gene Full Name: bone morphogenetic protein 4
• Alias Symbols: Bmp-;Bmp2;Bmp2b;Bmp2b-;Bmp2b1;Bmp2b-1;Bmp-4;bone morphogenetic protein 2B;bone morphogenetic protein 4.
• NCBI Gene Id: 12159
• Protein Name: Bone morphogenetic protein 4
• Description of Target: Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
• Uniprot ID: P21275
• Protein Accession #: NP_001303289
• Nucleotide Accession #: NM_001316360