ATP1B1 ELISA Kit (Mouse) (OKCD08924)

Catalog No: OKCD08924

Size:96WELLS

ATP1B1 ELISA Kit (Mouse) (OKCD08924) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ATP1B1 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP1B1 Lyophilized Standard: 2
  • 100X ATP1B1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.066ng/mL

Target Info

• Gene Symbol: ATP1B1
• Gene Full Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Alias Symbols: At, Atpb, Atp4b, Atpb-1, NKbeta1
• NCBI Gene Id: 11931
• Protein Name: sodium/potassium-transporting ATPase subunit beta-1
• Description of Target: This gene encodes an integral membrane protein that comprises a subunit of an ATP-metabolizing enzyme responsible for transporting sodium and potassium ions across the plasma membrane. This enzyme regulates the electrochemical gradient of these ions in cells, and plays a central role in osmoregulation and signal transmission in nerves and muscles, among other biological processes. The encoded protein is the non-catalytic beta subunit; it works together with a catalytic alpha subunit and a gamma subunit.
• Uniprot ID: P14094
• Protein Accession #: NP_033851.1
• Nucleotide Accession #: NM_009721.6