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ATP1B1 Antibody - middle region (ARP44411_P050)
Datasheets/Manuals | Printable datasheet for anti-ATP1B1 (ARP44411_P050) antibody |
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Tested Species Reactivity | Human, Rat |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | IHC, WB |
Additional Information | IHC Information: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. IHC Information: Paraffin embedded kidney tissue, tested with an antibody dilution of 5 ug/ml. |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human ATP1B1 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 92%; Dog: 86%; Guinea Pig: 85%; Horse: 86%; Human: 100%; Mouse: 100%; Pig: 92%; Rabbit: 92%; Rat: 100%; Sheep: 92% |
Peptide Sequence | Synthetic peptide located within the following region: VMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLL |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-ATP1B1 (ARP44411_P050) antibody is Catalog # AAP44411 (Previous Catalog # AAPS15909) |
Sample Type Confirmation | ATP1B1 is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
Subunit | beta-1 |
Reference | Vagin,O., (2005) J. Biol. Chem. 280 (52), 43159-43167 |
Publications | Dec1 and CLOCK Regulate Na+/K+-ATPase β1 Subunit Expression and Blood Pressure. Hypertension. 72, 746-754 (2018). 30012868 |
Description |
Gene Symbol | ATP1B1 |
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Gene Full Name | ATPase, Na+/K+ transporting, beta 1 polypeptide |
Alias Symbols | ATP1B |
NCBI Gene Id | 481 |
Protein Name | Sodium/potassium-transporting ATPase subunit beta-1 |
Description of Target | ATP1B1 belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit.The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. |
Uniprot ID | P05026 |
Protein Accession # | NP_001001787 |
Nucleotide Accession # | NM_001001787 |
Protein Size (# AA) | 301 |
Molecular Weight | 35kDa |
Protein Interactions | Bace1; EGFR; ATP4A; UBC; BRCA1; BARD1; PAXIP1; GCH1; USP4; NEDD4L; ELAVL1; NDRG2; TRMT2A; DDAH2; HLA-DRB1; HLA-DRA; EZH2; CRIP2; KMT2B; PSME1; HLA-DRB5; HLA-DRB4; HLA-DRB3; LRIF1; FXYD7; FXYD1; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Sheep".
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How long will it take to receive "ATP1B1 Antibody - middle region (ARP44411_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "ATP1B1 Antibody - middle region (ARP44411_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
This target may also be called "ATP1B" in publications.
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What is the shipping cost for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "35kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ATP1B1 Antibody - middle region (ARP44411_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATP1B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATP1B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATP1B1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATP1B1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATP1B1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATP1B1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.