ATF3 Antibody - middle region (ARP36556_P050)

Catalog No: ARP36556_P050

Host: Rabbit
Target Name: ATF3
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: ATF3
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: ATF3
Positive control (+): Human liver (LI)
Negative control (-): Stomach tumor (T-ST)
Antibody concentration: 3ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF3
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 93%; Rat: 93%; Yeast: 75%; Zebrafish: 80%
• Peptide Sequence: Synthetic peptide located within the following region: PLGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ATF3 (ARP36556_P050) antibody is Catalog # AAP36556 (Previous Catalog # AAPP07707)
• Reference: Sampson,M.J., (2008) Eur. J. Endocrinol. 158 (5), 729-739

Target Info

• Gene Symbol: ATF3
• Gene Full Name: Activating transcription factor 3
• Alias Symbols: -
• NCBI Gene Id: 467
• Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Description of Target: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
• Uniprot ID: P18847
• Protein Accession #: NP_001665
• Nucleotide Accession #: NM_001674
• Protein Size (# AA): 181
• Molecular Weight: 20kDa
• Protein Interactions: SUV39H1; BATF3; MAFF; CREB3; FOSL1; MAFG; JUNB; JUN; FOS; DDIT3; DBP; ATF2; CEBPG; CEBPE; CEBPA; ATF4; ATF3; BATF; HSP90AA1; SRA1; ZNF212; ID3; FHL2; SUMO1; SUMO3; HDAC10; HDAC5; HDAC6; HDAC4; HDAC3; HDAC1; SMARCA4; PDX1; ACTB; UBC; SUMO2; STAT1; MDM2; SM