Anti-p15INK4b Antibody (OAAI00734)

Catalog No: OAAI00734

Size:100UG

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase. The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a aploinsufficient tumor suppressor in vivo. All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6. The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. The gene encoding p15 INK4B is often silenced by hypermethylation in acute myeloid leukemia and deleted in acute lymphocytic leukemia.
  Positive Control: 3T3, Hela
  Cellular Location: Nucleus
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the C-terminus of Mouse p15INK4b. This sequence is identical to human, rat and mouse.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: KDKN2B
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested

Target Info

• Gene Symbol: CDKN2B
• Gene Full Name: cyclin dependent kinase inhibitor 2B
• Alias Symbols: CDK inhibitory protein;CDK4B inhibitor;CDK4I;cyclin-dependent kinase 4 inhibitor B;cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4);cyclin-dependent kinases 4 and 6 binding protein;INK4B;MTS2;MTS-2;multiple tumor suppressor 2;p14_CDK inhibitor;p14_INK4B;p14-INK4b;P15;p15 CDK inhibitor;p15_INK4B;p15INK4b;p15-INK4b;TP15.
• NCBI Gene Id: 1030
• Description of Target: Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase. The inhibitors of CDK4 (INK4) family include p15INK4B, p16INK4A, p18INK4C and p19INK4D. p18 has been shown to function as a aploinsufficient tumor suppressor in vivo. All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6. The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. The gene encoding p15INK4B is often silenced by hypermethylation in acute myeloid leukemia and deleted in acute lymphocytic leukemia.
• Molecular Weight: 15