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Catalog No: OKEH01367 (Formerly GWB-ZPB7RV)
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ANGPTL2 ELISA Kit (Human) (OKEH01367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPTL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPTL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPTL2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ANGPTL2 Microplate96 Wells (12 x 8 Well strips)
ANGPTL2 Lyophilized Standard2
100X Biotinylated ANGPTL2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.39 ng/mL
SpecificityNatural and recombinant Human Angiopoietin-related protein 2

Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer. Transl Oncol. 12, 539-544 (2019). 30611902

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolANGPTL2
Gene Full Nameangiopoietin like 2
Alias Symbolsangiopoietin-like protein 2;angiopoietin-related protein 2;ARP2;HARP.
NCBI Gene Id23452
Protein NameQ9UKU9
Description of TargetAngiopoietin-related protein 2
Uniprot ID
Protein Accession #NP_036230.1
Nucleotide Accession #NM_012098.2
Protein Size (# AA)493
Molecular Weight57 kDa
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