ANGPTL2 ELISA Kit (Human) (OKEH01367)

Catalog No: OKEH01367 (Formerly GWB-ZPB7RV)

Size:96 Wells

Human ANGPTL2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ANGPTL2 ELISA Kit (Human) (OKEH01367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPTL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPTL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPTL2 captured in well.
• ELISA Kit Range: 0.78 - 50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.4% (n = 20)
  Mean Inter-assay CV%: < 8.4% (n = 20)
• ELISA Kit Component:
  • ANGPTL2 Microplate: 96 Wells (12 x 8 Well strips)
  • ANGPTL2 Lyophilized Standard: 2
  • 100X Biotinylated ANGPTL2 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.39 ng/mL
• Specificity: Natural and recombinant Human Angiopoietin-related protein 2
• Publications:

  Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer. Transl Oncol. 12, 539-544 (2019). 30611902

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANGPTL2
• Gene Full Name: angiopoietin like 2
• Alias Symbols: ARP2, HARP
• NCBI Gene Id: 23452
• Protein Name: Angiopoietin-related protein 2
• Description of Target: Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
• Uniprot ID: Q9UKU9
• Protein Accession #: NP_036230.1
• Nucleotide Accession #: NM_012098.2
• Protein Size (# AA): 493
• Molecular Weight: 57 kDa