AIF (1B7) Mouse mAb (OAAH00022)

Catalog No: OAAH00022

Size:100 ul

Western blot analysis of AIF (1:2000) expression in HeLa (lane 1), 293T (lane 2), MCF7 (lane 3), COS7 (lane 4), NIH/3T3 (lane 5), PC12 (lane 6), CHO-K1 (lane 7), MDCK (lane 8)
Each lane was loaded with 20 ug whole cell lysate.

Product Info

• Predicted Species Reactivity: Human, Rat, Monkey, Chinese hamster, Dog
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Store the product at -20C. Stable for one year from the date of shipment.
• Immunogen: This Aviva monoclonal antibody is produced by immunizing mice with asynthetic peptide (KLH-coupled) corresponding to residues within the carboxy terminus of AIF.
• Specificity: AIF (1B7) Mouse mAb detects endogenous levels of total AIF protein.
• Source: Mouse mAb
• Application Info: WB: 1:2000
• Reference: 1. Zamzami, N., et al. 1996. Mitochondrial control of nuclear apoptosis. J. Exp.
  Med. 183: 1533-1544.
  2. Susin, S.A., et al. 1996. Bcl-2 inhibits the mitochondrial release of an
  apoptogenic protease. J. Exp. Med. 184: 1331-1341.
  3. Kluck, R.M., et al. 1997. The release of cytochrome c from mitochondria: a
  primary site for Bcl-2 regulation of apoptosis. Science 275: 1132-1136.
  4. Green, D.R., et al. 1998. Mitochondria and apoptosis. Science 281: 1309-
  1312.
  5. Mancini, M., et al. 1998. The caspase-3 precursor has a cytosolic and
  mitochondrial distribution: implications for apoptotic signaling. J. Cell. Biol.
  140: 1485-1495.
  6. Susin, S.A., et al. 1999. Mitochondrial release of caspase-2 and -9 during
  the apoptotic process. J. Exp. Med. 189: 381-394.
  7. Susin, S.A., et al. 1999. Molecular characterization of mitochondrial
  apoptosis-inducing factor. Nature 397: 441-446.

Target Info

• Gene Symbol: AIFM1
• Alias Symbols: AIF, AUNX1, CMT2D, CMTX4, COWCK, DFNX5, NADMR, NAMSD, PDCD8, COXPD6, SEMDHL
• NCBI Gene Id: 9131
• Protein Name: Apoptosis-inducing factor 1, mitochondrial
• Description of Target: A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9 and AIF (apoptosis-inducing factor) . AIF was shown to cause DNA fragmentation and chromatin condensation, and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression was shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.
• Uniprot ID: O95831
• Molecular Weight: 67 kDa