ADAM10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03691)

Catalog No: OKCD03691

Size:96WELLS

Rat ADAM10 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-98%	91-98%	95-103%	92-101%
  EDTA Plasma (n=5)	93-102%	84-92%	98-105%	85-104%
  Heparin Plasma (n=5)	86-101%	98-105%	86-104%	81-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the A Disintegrin And Metalloprotease 10 (ADAM10) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	99-105	102
  EDTA Plasma (n=5)	96-104	101
  Heparin Plasma (n=5)	79-93	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Adam10 Microplate: 96 Wells (12 x 8 Well strips)
  • Adam10 Lyophilized Standard: 2
  • 100X Adam10 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Adam10
• Gene Full Name: ADAM metallopeptidase domain 10
• Alias Symbols: a disintegrin and metallopeptidase domain 10;a disintegrin and metalloprotease domain 10;ADAM 10;disintegrin and metalloproteinase domain-containing protein 10;kuzbanian protein homolog;MADM;mammalian disintegrin-metalloprotease;RGD1566370.
• NCBI Gene Id: 29650
• Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Description of Target: Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.
• Uniprot ID: Q10743
• Protein Accession #: XP_001054737
• Nucleotide Accession #: XM_001054737