AANAT ELISA Kit (Human) (OKCD07816)

Catalog No: OKCD07816

Size:96WELLS

AANAT ELISA Kit (Human) (OKCD07816) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylalkylamine-N-Acetyltransferase (AANAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylalkylamine-N-Acetyltransferase (AANAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylalkylamine-N-Acetyltransferase (AANAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arylalkylamine-N-Acetyltransferase (AANAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arylalkylamine-N-Acetyltransferase (AANAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arylalkylamine-N-Acetyltransferase (AANAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AANAT Microplate: 96 Wells (12 x 8 Well strips)
  • AANAT Lyophilized Standard: 2
  • 100X AANAT HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.35ng/mL

Target Info

• Gene Symbol: AANAT
• Gene Full Name: aralkylamine N-acetyltransferase
• Alias Symbols: Aralkylamine N-acetyltransferase;arylalkylamine N-acetyltransferase;DSPS;serotonin acetylase;serotonin N-acetyltransferase;SNAT.
• NCBI Gene Id: 15
• Protein Name: Serotonin N-acetyltransferase
• Description of Target: Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
• Uniprot ID: Q16613
• Protein Accession #: NP_001079.1
• Nucleotide Accession #: NM_001088.2