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Catalog No: OKEH01379
Size:96T
Price: $600.00
SKU
OKEH01379
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AANAT ELISA Kit (Rat) (OKEH01379) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AANAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AANAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AANAT captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
AANAT Microplate96 Wells (12 x 8 Well strips)
AANAT Lyophilized Standard2
100X Biotinylated AANAT Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.186 ng/mL
SpecificityNatural and recombinant Rat Serotonin N-acetyltransferase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAanat
Gene Full Namearalkylamine N-acetyltransferase
Alias SymbolsAA-NAT;Aralkylamine N-acetyltransferase;arylakylamine N-acetyltransferase;Arylalkylamine N - acetyltransferase (Serotonin N - acetyltransferase);arylalkylamine N-acetyltransferase;Nat4;Seretonin N-acetyltransferase;serotonin acetylase;serotonin N-acetyltransferase.
NCBI Gene Id25120
Protein NameQ64666
Description of TargetSerotonin N-acetyltransferase
Uniprot IDhttps://www.uniprot.org/uniprot/Q64666
Protein Accession #NP_036950.1
Nucleotide Accession #NM_012818.2
Protein Size (# AA)205
Molecular Weight23 kDa
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