YARS Protein (OOPA00144)
|Product Format||Sterile Filtered colorless solution.|
|::||Product Introduction: Tyrosyl-tRNA synthetase (YARS) is a member of the class I tRNA synthetase family. YARS is a vital enzyme which catalyzes the aminoacylation of tRNATrp with tryptophan, a critical function of the cell .s protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma.
Product Description: YARS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 548 amino acids (1-528 a.a.) and having a molecular mass of 61.3kDa. YARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
|Reconstitution and Storage||YARS Human Recombinant although stable at 4C for 1 week, should be stored below -18C. Please prevent freeze thaw cycles.|
|Purity||Greater than 90.0% as determined by SDS-PAGE.|
|Peptide Sequence||MGSSHHHHHH SSGLVPRGSH MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS.|
|Formulation||YARS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.|
|Datasheets/Manuals||Printable datasheet for OOPA00144|
|Purification||Greater than 90.0% as determined by SDS-PAGE.|
|Alias Symbols||Tyrosyl-tRNA synthetase cytoplasmic, Tyrosyl--tRNA ligase, TyrRS, YARS, YRS, YTS, CMTDIC.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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