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Datasheets/Manuals | Printable datasheet for XRCC5 Antibody (OACD08299) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Range: Leu251 - Ile732 |
Purification | Affinity Purified; Protein A Purified |
Concentration | 0.5 mg/ml |
Preservative | 0.02% NaN3 |
Specificity | The antibody is a rabbit polyclonal antibody raised against XRCC5. It has been selected for its ability to recognize XRCC5 in immunohistochemical staining and western blotting. |
Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
Gene Symbol | Xrcc5 |
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Gene Full Name | X-ray repair complementing defective repair in Chinese hamster cells 5 |
Alias Symbols | AI314015;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair protein XRCC5;ku autoantigen protein p86 homolog;Ku p80;Ku80;Ku86;nuclear factor IV;X-ray repair cross-complementing protein 5. |
NCBI Gene Id | 22596 |
Protein Name | X-ray repair cross-complementing protein 5 |
Description of Target | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. |
Uniprot ID | P27641 |
Protein Accession # | NP_033559.2 |
Nucleotide Accession # | NM_009533 |
Molecular Weight | 83 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "XRCC5 Antibody (OACD08299)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "XRCC5 Antibody (OACD08299)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "XRCC5 Antibody (OACD08299)" provided in?
This item is provided in "Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "XRCC5 Antibody (OACD08299)"?
This target may also be called "AI314015;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair protein XRCC5;ku autoantigen protein p86 homolog;Ku p80;Ku80;Ku86;nuclear factor IV;X-ray repair cross-complementing protein 5." in publications.
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What is the shipping cost for "XRCC5 Antibody (OACD08299)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "XRCC5 Antibody (OACD08299)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "XRCC5 Antibody (OACD08299)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "83 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "XRCC5 Antibody (OACD08299)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Xrcc5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Xrcc5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Xrcc5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Xrcc5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Xrcc5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Xrcc5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.