WTIP ELISA Kit (Human) (OKEH04763)

Catalog No: OKEH04763

Size:96T

Human WTIP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology WTIP ELISA Kit (Human) (OKEH04763) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WTIP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WTIP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WTIP captured in well.
• ELISA Kit Range: 0.39-25ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • WTIP Microplate: 96 Wells (12 x 8 Well Strips)
  • WTIP Standard: 2
  • Sample Diluent: 1 x 20 mL
  • WTIP Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.098 ng/mL
• Specificity: Natural and recombinant Human Wilms tumor protein 1-interacting protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: WTIP
• Gene Full Name: WT1 interacting protein
• Alias Symbols: Wilms tumor 1 interacting protein;Wilms tumor protein 1-interacting protein.
• NCBI Gene Id: 126374
• Protein Name: A6NIX2
• Description of Target: Wilms tumor protein 1-interacting protein
• Uniprot ID: https://www.uniprot.org/uniprot/A6NIX2
• Protein Accession #: NP_001073905.1
• Nucleotide Accession #: NM_001080436.1
• Protein Size (# AA): 430
• Molecular Weight: 45 kDa