WNT11 Antibody (OAGA00450)

Catalog No: OAGA00450

Size:100 ul

Sample(30 ug whole cell lysate)
A:Hep G2
10% SDS PAGE
OAGA00450 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
OAGA00450 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11(OAGA00450) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11(OAGA00450) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. [provided by RefSeq]
  Positive Controls: HepG2, mouse heart
  Cellular Localization: Secreted, extracellular space, extracellular matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 310 of WNT11 (Uniprot ID#O96014)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: WNT11
• Gene Full Name: Protein Wnt-11
• Alias Symbols: HWNT11
• NCBI Gene Id: 7481
• Protein Name: Protein Wnt-11
• Uniprot ID: O96014
• Protein Accession #: NP_004617.2
• Molecular Weight: 39 kDa