Wnt10b Antibody (OAPB00686)

Catalog No: OAPB00686

Size:100UG

Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 ug/mL.

Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Wnt10b antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b. The immunogen is located within amino acids 130 - 180 of Wnt10b.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Wnt10b antibody is Catalog # OEPB02799
• Application Info: Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 ug/ml. (Optimal dilution should be determined by user)
• Reference: Lee FS, Lane TF, Kuo A, et al. Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice. Proc. Natl. Acad. Sci. USA1995; 92:2268-72.;Nusse R and Varmus RE. Wnt genes. Cell1992; 69:1073-87.;Bui TD, Rankin J, Smith K, et al. A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene1997; 14:1249-53.;Bennett CN, Hodge CL, MacDougald OA, et al. Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochem. Biophys. Res. Commun.2003; 302:12-16.

Target Info

• Gene Symbol: WNT10B
• Gene Full Name: Wnt family member 10B
• Alias Symbols: protein Wnt-10b;Protein Wnt-12;SHFM6;STHAG8;wingless-type MMTV integration site family, member 10B;WNT-10B protein;WNT-12.
• NCBI Gene Id: 7480
• Protein Name: Protein Wnt-10b|protein Wnt-10b precursor [Homo sapiens]
• Description of Target: Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
• Uniprot ID: O00744
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_003385
• Molecular Weight: Predicted: 43 kDa Observed: 47 kDa