WDR82 Antibody - N-terminal region (OAAB18938)

Catalog No: OAAB18938

Size:400ul

Western blot analysis of lysates from NCCIT, SH-SY5Y cell line (from left to right), using WDR82 Antibody (N-term) (Cat # OAAB18938). OAAB18938 was diluted at 1: 1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Fluorescent image of Hela cells stained with WDR82 Antibody (N-term) (Cat # OAAB18938). OAAB18938 was diluted at 1: 25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1: 400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P-Leica, WB, ELISA
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:2000
  IHC-P-Leica: 1:500
• Reference: Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  Lee J.-H.,et al.J. Biol. Chem. 280:41725-41731(2005).
  Higa L.A.,et al.Nat. Cell Biol. 8:1277-1283(2006).
  

Target Info

• Gene Symbol: WDR82
• Alias Symbols: SWD2, MST107, WDR82A, MSTP107, PRO2730, TMEM113, PRO34047
• NCBI Gene Id: 80335
• Description of Target: Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
• Uniprot ID: Q6UXN9; Q8BFQ4
• Molecular Weight: 35 kDa