VNN3 ELISA Kit (Human) (OKCD02982)

Catalog No: OKCD02982

Size:96WELLS

Human VNN3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-103%	88-101%	80-91%	82-92%
  EDTA Plasma (n=5)	93-105%	83-92%	83-96%	93-103%
  Heparin Plasma (n=5)	80-97%	90-97%	88-102%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vanin 3 (VNN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vanin 3 (VNN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vanin 3 (VNN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vanin 3 (VNN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-91	80
  EDTA Plasma (n=5)	78-102	97
  Heparin Plasma(n=5)	96-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vanin 3 (VNN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vanin 3 (VNN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-VNN3 Microplate: 96 Wells (12 x 8 Well strips)
  • VNN3 Lyophilized Standard: 2
  • 100X Biotinylated VNN3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vanin 3 (VNN3). No significant cross-reactivity or interference between Vanin 3 (VNN3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: VNN3P
• Gene Full Name: vanin 3, pseudogene
• Alias Symbols: HSA238982;pantetheinase pseudogene;pantetheinase-associated gene expressed in leukocytes (PAGEL)-alpha;vanin 1 pseudogene;vascular non-inflammatory molecule 3;VNN3.
• NCBI Gene Id: 55350
• Protein Name: Vascular non-inflammatory molecule 3
• Description of Target: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
• Uniprot ID: Q9NY84
• Protein Accession #: NP_001278632.1
• Nucleotide Accession #: NM_001291703.1
• Protein Size (# AA): 501