VINCULIN Antibody (OAAD00213)

Catalog No: OAAD00213

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Chicken
• Clonality: Monoclonal
• Clone: VIN-54
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Human vinculin, purified from uterus.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat, chicken.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 1-2ug/ml with the appropriate system to detect vinculin in cells and tissues.
  Immunohistochemistry(F) At 2-4ug/ml to detect vinculin in formalin or acetone fixed tissues.
  Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Weller, P. A.; Ogryzko, E. P.; Corben, E. B.; Zhidkova, N. I.; Patel, B.; Price, G. J.; Spurr, N. K.; Koteliansky, V. E.; Critchley, D. R. : Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc. Nat. Acad. Sci. 87: 5667-5671, 1990.
  2. Mulligan, L. M.; Gardner, E.; Telenius, H.; Ponder, B. A. J. : Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q. Genomics 13: 1347-1349, 1992.

Target Info

• Gene Symbol: VCL
• Alias Symbols: MV, MVCL, CMD1W, CMH15, HEL114
• NCBI Gene Id: 7414
• Protein Name: Vinculin
• Description of Target: Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1, 066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
• Uniprot ID: P18206