Uricase Protein (OPPA00832)

Catalog No: OPPA00832 (Formerly GWB-4DA0F6)

Size:100UG

Product Info

• Product Format: Lyophilized 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6 mg Na₂HPO₄*2H₂0 and 0.33 mg NaH2PO4*2H20.
• Host: E. Coli
• Additional Information: Molecular Formula: C₁₅₂₃H₂₃₈₃N₄₁₇O₄₆₂S₇
• Reconstitution and Storage: We highly recommend reconstituting the lyophilized Uricase in 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH 8.5 for activity assay.
  Lyophilized Urate Oxidase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Uricase should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Biological Activity: The specific activity was found to be 10U/mg.
  One Unit oxidizes one micromole of uric acid per minute at 25°C, at pH 8.5.
• Protein Sequence: msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl

Target Info

• Gene Symbol: Uricase
• Alias Symbols: Uri, Uri2, UOX-2
• NCBI Gene Id: 114768
• Protein Name: Uricase
• Description of Target: Urate oxidase catalyzes the enzymatic oxidation (degrades) of uric acid into allantoin, an inactive and soluble metabolite, which is 5 to 10 fold more soluble than uric acid . Urate oxidase is an enzyme of the purine breakdown pathway that catalyses the oxidation of uric acid to allantoin. Uricase is present in numerous diverse organisms, but not in higher primates including human. Hyperuricaemia is most commonly associated with gout and also occurs in mammalians with malignancy, especially those with lymphoid malignancies due to rapid cell turnover and an increased rate of purine metabolism. Uricase is effective in the prevention and treatment of hyperuricaemia in mammalians with malignancy and in those who have undergone transplantation. It appears to act rapidly, safely and induces a more dramatic decrease in plasma levels of uric acid. The cDNA coding for urate-oxidase was cloned from a strain of Aspergillus flavus. The monomer protein has no intra- or inter-disulfide bridges.
• Uniprot ID: P09118
• Protein Accession #: NP_446220.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 34 kDa