UNC13D Antibody (OAGA00643)

Catalog No: OAGA00643

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Jurkat
B: K562
5% SDS PAGE
OAGA00643 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Stomach, using UNC13D(OAGA00643) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using Munc 13-4(OAGA00643) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder. [provided by RefSeq]
  Positive Controls: Jurkat, K562
  Cellular Localization: Cytoplasm, Membrane; Peripheral membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1029 and 1090 of Munc 13-4 (Uniprot ID#Q70J99)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UNC13D
• Gene Full Name: Protein unc-13 homolog D
• Alias Symbols: FHL3, HLH3, HPLH3, Munc13-4
• NCBI Gene Id: 201294
• Protein Name: Protein unc-13 homolog D
• Uniprot ID: Q70J99
• Protein Accession #: NP_954712.1
• Molecular Weight: 123 kDa