UFD1L Antibody (OAGA00777)

Catalog No: OAGA00777

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGEOAGA00777 diluted at 1:10000

UFD1L antibody detects UFD1L protein by Western blot analysis.
A. 50 ug mouse lung lysate/extract
12 % SDS-PAGE
UFD1L antibody (OAGA00777) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L(OAGA00777) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L(OAGA00777) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, that is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
  Positive Controls: A431, H1299, mouse lung
  Cellular Localization: Nucleus (By similarity), Cytoplasm, cytosol
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 307 of UFD1L (Uniprot ID#Q92890)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1.02 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UFD1L
• Gene Full Name: Ubiquitin fusion degradation protein 1 homolog
• Alias Symbols: UFD1L
• NCBI Gene Id: 7353
• Protein Name: Ubiquitin fusion degradation protein 1 homolog
• Uniprot ID: Q92890
• Protein Accession #: NP_005650.2
• Molecular Weight: 35 kDa