UCP3 Antibody (OAGA04096)

Catalog No: OAGA04096

Size:100 ul

UCP3 antibody detects UCP3 protein by Western blot analysis.
A. 30 ug RD whole cell lysate/extract
12 % SDS-PAGE
UCP3 antibody dilution: 1:1000

UCP3 antibody detects UCP3 protein by Western blot analysis.
A. 50 ug rat muscle lysate/extract
12 % SDS-PAGE
UCP3 antibody dilution: 1:500

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Full length human UCP3 Recombinant protein.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.66 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: UCP3
• Gene Full Name: uncoupling protein 3
• Alias Symbols: SLC25A9
• NCBI Gene Id: 7352
• Protein Name: mitochondrial uncoupling protein 3
• Description of Target: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.
• Uniprot ID: P55916
• Protein Accession #: NP_003347.1
• Nucleotide Accession #: NM_003356.3