TYROBP ELISA Kit (Bovine) (OKEH07870)

Catalog No: OKEH07870

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology TYROBP ELISA Kit (Bovine) (OKEH07870) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TYROBP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TYROBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TYROBP captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.6% (n = 20)
  Mean Inter-assay CV%: <=10.0% (n = 20)
• ELISA Kit Component:
  • TYROBP Microplate: 96 Wells (12 x 8 Well strips)
  • TYROBP Lyophilized Standard: 2
  • 100X Biotinylated TYROBP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.
  Subcellular Location: Membrane Single-pass type I membrane protein
  Unigene: Bt.8939
  SMR: Q95J79
  String: 9913.ENSBTAP00000009652
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine TYRO protein tyrosine kinase-binding protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TYROBP
• Gene Full Name: transmembrane immune signaling adaptor TYROBP
• Alias Symbols: dap12;DNAX-activation protein 12;TYRO protein tyrosine kinase binding protein;TYRO protein tyrosine kinase-binding protein.
• NCBI Gene Id: 282390
• Protein Name: Q95J79
• Description of Target: TYRO protein tyrosine kinase-binding protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q95J79
• Protein Accession #: XP_005218963.1
• Nucleotide Accession #: XM_005218906.3