TYR ELISA Kit (Dog) (OKCA02230)

Catalog No: OKCA02230

Size:96 Wells

Dog TYR ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	94	91-99
  1:2	92	88-96
  1:4	88	83-96
  1:8	97	91-103

• ELISA Kit Principle: Aviva Systems Biology TYR ELISA Kit (Dog) (OKCA02230) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TYR has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TYR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard TYR captured in the well.
• ELISA Kit Range: 31.2 - 2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	95	89-98
  EDTA Plasma (n=4)	88	82-94

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • TYR Microplate: 96 Wells (12 x 8 Well strips)
  • TYR Lyophilized Standard: 2
  • 100X Biotinylated TYR Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TYR
• Alias Symbols: OCA1A, OCAIA, SHEP3, tyrosinase
• NCBI Gene Id: 403405
• Protein Name: Tyrosinase
• Description of Target: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
• Uniprot ID: P54834
• Protein Accession #: NP_001002941
• Nucleotide Accession #: NM_001002941