TUBB8 Antibody (OAGA00785)

Catalog No: OAGA00785

Size:100 ul

Sample(30 ug whole cell lysate)
A:A431
B:H1299
10% SDS PAGE
OAGA00785 diluted at 1:1000

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 whole cell lysate/extract
F. 30 ug Raw264.7 whole cell lysate/extract
G. 30 ug C2C12 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 8 antibody [C2C3], C-term (OAGA00785) dilution: 1:1000

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
beta Tubulin 8 antibody [C2C3], C-term (OAGA00785) dilution: 1:500.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8(OAGA00785) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments.
IP Sample: 293T whole cell lysate/extract
A. 40 ?g 293T whole cell lysate/extract
B. Control with 2 ?g of preimmune rabbit IgG
C. Immunoprecipitation of beta Tubulin 8 protein by 2 ?g of beta Tubulin 8 antibody (OAGA00785)
10% SDS-PAGE
The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody (OAGA00785) diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, IP, WB
• Additional Information: Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  Positive Controls: A431, H1299, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 366 and 444 of beta Tubulin 8 (Uniprot ID#Q3ZCM7)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TUBB8
• Gene Full Name: Tubulin beta-8 chain
• Alias Symbols: OOMD, OOMD2, bA631M21.2
• NCBI Gene Id: 347688
• Protein Name: Tubulin beta-8 chain
• Uniprot ID: Q3ZCM7
• Protein Accession #: NP_817124.1
• Molecular Weight: 50 kDa