TUBA4A Antibody - middle region (ARP40179_P050)

Catalog No: ARP40179_P050

WB Suggested Anti-TUBA4A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Placenta

Sample Type: 1. Human NT-2 cells (60ug)
2. and 3. mouse brain extracts (80ug)
4. rat brain extract (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science 

Sample Type:
Mouse brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
TUBA4A: Red DAPI:Blue
Gene Name:
TUBA4A
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Amount and Sample Type:
500 ug mouse brain homogenate
Amount of IP Antibody:
6 ug
Primary Antibody:
TUBA4A
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
TUBA4A
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, IP, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TUBA4A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%
• Peptide Sequence: Synthetic peptide located within the following region: YAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-TUBA4A (ARP40179_P050) antibody is Catalog # AAP40179 (Previous Catalog # AAPP22025)
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Other Applications Image 1 Data: IP Suggested Anti-TUBA4A Antibody
  Positive Control: NT2 CELL/BRAIN TISSUE
  
• Reference: Fiore,G., (2006) Neurosci. Lett. 394 (1), 57-62
• Other Applications Image 2 Data: IP Suggested Anti-TUBA4A antibody
  Titration: 2 ug/ml
  Positive Control: Mouse brain homogenate
  
• Description:
  View product citations for TUBA4A Antibody - middle region (ARP40179_P050) on CiteAb

Target Info

• Gene Symbol: TUBA4A
• Gene Full Name: Tubulin, alpha 4a
• Alias Symbols: ALS22, TUBA1, H2-ALPHA
• NCBI Gene Id: 7277
• Protein Name: Tubulin alpha-4A chain
• Description of Target: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P68366
• Protein Accession #: NP_005991
• Nucleotide Accession #: NM_006000
• Protein Size (# AA): 448
• Molecular Weight: 50 kDa
• Protein Interactions: MAPT; CDC20; HAUS1; CEP76; HAUS2; MAGED2; TUBGCP2; CEP57; UBC; TUBG1; TP53; SUMO2; STAU1; MDM2; MGRN1; RPA3; RPA2; RPA1; rev; TUBB; TUBA1C; TUBB6; TUBA4B; TUBB4B; TUBA1A; TXNRD1; TUBB2A; RPS27A; PRPS1; YBX1; HSPA5; HSPA1A; CRYZ; PARK2; EGFR; SOX2; ICAM1;