TPI1 Antibody (OAEB01304)

Catalog No: OAEB01304 (Formerly GWB-9100A3)

Size:100UG

OAEB01304 staining (0.003ug/ml) of human liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB01304 (2ug/ml) staining of paraffin embedded Human Liver Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC, WB
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: TPI1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Guinea Pig: 100%; Human: 100%; Yeast: 100%
• Peptide Sequence: C-LKPEFVDIINAKQ
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:16000.
  Western Blot: Approx 26kDa band observed in human liver and human hepatoblastoma HepG2 cell lysates (calculated MW of 26.7kDa according to NP_000356). Recommended concentration: 0.01-0.03ug/ml.
• Reference: * Jorge I, Navarro P, Martinez-Acedo P, N E, Serrano H, Alfranca A, Redondo JM, Vazquez J.; Statistical model to analyze quantitative proteomics data obtained by 18O/16O labeling and linear ion trap mass spectrometry: application to the study of vascular endothelial growth factor-induced angiogenesis in endothelial cells.; Mol Cell Proteomics. 2009 May;8(5):1130-49.; Pubmed 19181660
  * Kim JE, Koo KH, Kim YH, Sohn J, Park YG.; Identification of potential lung cancer biomarkers using an in vitro carcinogenesis model.; Exp Mol Med. 2008 Dec 31;40(6):709-20.; Pubmed 19116456
  * Honore B, Buus S, Claesson MH.; Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.; Proteome Sci. 2008 Jun 10;6:18.; Pubmed 18544163
  * O'Connell K, Gannon J, Doran P, Ohlendieck K.; Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle.; Med. 2007 Aug;20(2):145-53.; Pubmed 17611631
  * Vorum H, ostergaard M, Rice GE, Honore B, Bek T.; Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study.; Proteome Sci. 2007 Feb 27;5:5.; Pubmed 17326818
  * Jiang PZ, Gan M, Huang H, Shen XM, Wang S, Yao KT.; Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells.; Acta Biochim Biophys Sin (Shanghai). 2005 Feb;37(2):97-106.; Pubmed 15685366

Target Info

• Gene Symbol: Tpi1|TPI1
• Gene Full Name: triosephosphate isomerase 1
• Alias Symbols: AI255506;epididymis secretory protein Li 49;HEL-S-49;methylglyoxal synthase;TIM;Tpi;Tpi-1;TPID;triosephosphate isomerase;triose-phosphate isomerase;triosephosphate isomerase-like.
• NCBI Gene Id: 21991|24849|7167
• Protein Name: Triosephosphate isomerase
• Uniprot ID: P60174
• Protein Accession #: NP_000356.1
• Molecular Weight: 26.7; 30.8; 18.0