TNFSF11 Antibody (OAAF02004)

Catalog No: OAAF02004 (Formerly GWB-ASD278)

Size:100 ug

Western blot analysis of extracts from HuvEc cells, using TNFSF11 Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using TNFSF11 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human TNFSF11.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: TNF11 antibody detects endogenous levels of total TNF11 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:40000
• Reference: Anderson D.M., Nature 390:175-179(1997).
  Lacey D.L., Cell 93:165-176(1998).
  Nagai M., Biochem. Biophys. Res. Commun. 269:532-536(2000).

Target Info

• Gene Symbol: TNFSF11
• Gene Full Name: TNF superfamily member 11
• Alias Symbols: CD254;hRANKL2;ODF;OPGL;OPTB2;osteoclast differentiation factor;osteoprotegerin ligand;RANKL;receptor activator of nuclear factor kappa B ligand;Receptor activator of nuclear factor kappa-B ligand;sOdf;TNF-related activation-induced cytokine;TNLG6B;TRANCE;tumor necrosis factor (ligand) superfamily, member 11;tumor necrosis factor ligand 6B;tumor necrosis factor ligand superfamily member 11;tumor necrosis factor superfamily member 11.
• NCBI Gene Id: 8600
• Protein Name: Tumor necrosis factor ligand superfamily member 11
• Description of Target: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
• Uniprot ID: O14788
• Protein Accession #: NP_003692.1
• Molecular Weight: 35 kDa