TNC ELISA Kit (Human) (OKCD07861)

Catalog No: OKCD07861

Size:96WELLS

TNC ELISA Kit (Human) (OKCD07861) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tenascin C (TNC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tenascin C (TNC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tenascin C (TNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tenascin C (TNC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tenascin C (TNC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tenascin C (TNC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TNC Microplate: 96 Wells (12 x 8 Well strips)
  • TNC Lyophilized Standard: 2
  • 100X TNC HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.1pg/mL

Target Info

• Gene Symbol: TNC
• Gene Full Name: tenascin C
• Alias Symbols: 150-225;cytotactin;deafness, autosomal dominant 56;DFNA56;glioma-associated-extracellular matrix antigen;GMEM;GP;GP 150-225;Hexabrachion;hexabrachion (tenascin);HXB;JI;myotendinous antigen;neuronectin;tenascin;Tenascin-C;tenascin-C additional domain 1;TN;TN-C.
• NCBI Gene Id: 3371
• Protein Name: Tenascin
• Description of Target: Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).
• Uniprot ID: P24821
• Protein Accession #: NP_002151.2
• Nucleotide Accession #: NM_002160.3