TJP1 ELISA Kit (Mouse) (OKEH08788)

Catalog No: OKEH08788

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology TJP1 ELISA Kit (Mouse) (OKEH08788) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TJP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TJP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TJP1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.5% (n = 20)
  Mean Inter-assay CV%: <=11.5% (n = 20)
• ELISA Kit Component:
  • TJP1 Microplate: 96 Wells (12 x 8 Well strips)
  • TJP1 Lyophilized Standard: 2
  • 100X Biotinylated TJP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.
  Subcellular Location: Cell membrane Peripheral membrane protein Cytoplasmic side Cell junction Tight junction Cell junction Gap junction Cytoplasm Myofibril Sarcomere I band Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells (By similarity).
  Unigene: Mm.4342
  SMR: P39447
  String: 10090.ENSMUSP00000099652
  KEGG: mmu:21872
  Pfam: PF00625
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.42ng/mL
• Specificity: Natural and recombinant Mouse Tight junction protein ZO-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Tjp1
• Gene Full Name: tight junction protein 1
• Alias Symbols: Tight junction protein 1;tight junction protein ZO-1;ZO;ZO-;ZO1;zona occludens protein 1;zonula occludens protein 1.
• NCBI Gene Id: 21872
• Protein Name: P39447
• Description of Target: Tight junction protein ZO-1
• Uniprot ID: https://www.uniprot.org/uniprot/P39447
• Protein Accession #: NP_033412.2
• Nucleotide Accession #: NM_009386.2