TICAM1 Antibody - C-terminal region (OABB01048)

Catalog No: OABB01048

Size:100UG

Anti-TRIF antibody, OABB01048, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: HL-60 Cell Lysate

Anti-TRIF antibody, OABB01048, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TICAM1, 44.7 kDa (162aa tag+ Q468-E712)
Lane 1: Recombinant Human TICAM1 Protein 10ng
Lane 2: Recombinant Human TICAM1 Protein 5ng
Lane 3: Recombinant Human TICAM1 Protein 2.5ng
Lane 4: Recombinant Human TICAM1 Protein 1.25ng

Anti-TRIF antibody, OABB01048, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for TICAM1 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: TICAM(TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TICAM1 or TRIF, is an adapter in responding to activation of toll-like receptors(TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering(si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for TIR domain-containing adapter molecule 1(TICAM1) detection. Tested with WB, IHC-P, IHC-F in Human.

Target Info

• Gene Symbol: TICAM1
• Gene Full Name: toll like receptor adaptor molecule 1
• Alias Symbols: IIAE6;MyD88-3;proline-rich, vinculin and TIR domain-containing protein B;PRVTIRB;putative NF-kappa-B-activating protein 502H;TICAM-1;TIR domain containing adaptor inducing interferon-beta;TIR domain-containing adapter molecule 1;TIR domain-containing adapter protein inducing IFN-beta;toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TRIF.
• NCBI Gene Id: 148022
• Protein Name: TIR domain-containing adapter molecule 1
• Description of Target: Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
• Uniprot ID: Q8IUC6
• Molecular Weight: 76422 MW