THSD7A ELISA Kit (Human) (OKCD04532)

Catalog No: OKCD04532

Size:96WELLS

THSD7A ELISA Kit (Human) (OKCD04532) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • THSD7A Microplate: 96 Wells (12 x 8 Well strips)
  • THSD7A Lyophilized Standard: 2
  • 100X THSD7A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.22ng/mL

Target Info

• Gene Symbol: THSD7A
• Gene Full Name: thrombospondin type 1 domain containing 7A
• Alias Symbols: thrombospondin type-1 domain-containing protein 7A;thrombospondin, type I, domain containing 7A.
• NCBI Gene Id: 221981
• Protein Name: Thrombospondin type-1 domain-containing protein 7A
• Description of Target: Thrombospondin type-1 domain-containing protein 7A: Plays a role in actin cytoskeleton rearrangement.
• Uniprot ID: Q9UPZ6
• Protein Accession #: NP_056019.1
• Nucleotide Accession #: NM_015204.2