TGM2 ELISA Kit (Human) (OKCD07709)

Catalog No: OKCD07709

Size:96WELLS

TGM2 ELISA Kit (Human) (OKCD07709) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 2 (TGM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 2 (TGM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 2 (TGM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 2 (TGM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 2 (TGM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 2 (TGM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TGM2 Microplate: 96 Wells (12 x 8 Well strips)
  • TGM2 Lyophilized Standard: 2
  • 100X TGM2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
• Sensitivity: < 0.229 ng/mL

Target Info

• Gene Symbol: TGM2
• Gene Full Name: transglutaminase 2
• Alias Symbols: C polypeptide, protein-glutamine-gamma-glutamyltransferase;erythrocyte transglutaminase;G(h);heart G alpha(h);hhG alpha(h);hTG2;isopeptidase TGM2;protein G alpha(h);protein-glutamine deamidase TGM2;protein-glutamine dopaminyltransferase TGM2;protein-glutamine gamma-glutamyltransferase 2;protein-glutamine histaminyltransferase TGM2;protein-glutamine noradrenalinyltransferase TGM2;protein-glutamine serotonyltransferase TGM2;TG(C);TGase C;TGase H;TGase II;TGase-2;TGC;tissue transglutaminase;transglutaminase C;transglutaminase H;transglutaminase II;Transglutaminase-2;tTG;tTgase.
• NCBI Gene Id: 7052
• Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Description of Target: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
• Uniprot ID: P21980
• Protein Accession #: NP_001310245.1
• Nucleotide Accession #: NM_001323316.1