Catalog No: OKEH02612
Size:96T
Price: $625.00
SKU
OKEH02612
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Tetrahydrobiopterin ELISA Kit (OKEH02612) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Tetrahydrobiopterin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tetrahydrobiopterin and incubated. The Tetrahydrobiopterin found in the sample or standards competes with the biotinylated Tetrahydrobiopterin for limited binding sites on the immobilized anti-Tetrahydrobiopterin antibody. Excess unbound biotinylated Tetrahydrobiopterin and sample or standard Tetrahydrobiopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tetrahydrobiopterin captured in the well and inversely proportional to the amount of Tetrahydrobiopterin which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/ml | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 100% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.9% (n = 20) Mean Inter-assay CV%: < 8.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: THB Target Name: Tetrahydrobiopterin | ||||||||||||||||||||||
:: | Pubchem: 44257 | ||||||||||||||||||||||
:: | Chemical formula: C9H15N5O3 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.067 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General Tetrahydrobiopterin | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | THB, Tetrahydrobiopterin, THB, BH4 |
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Protein Name | Tetrahydrobiopterin |
Description of Target | Tetrahydrobiopterin (BH4, THB, trade name Kuvan) or sapropterin (INN) is a naturally occurring essential cofactor of the three aromatic amino acid hydroxylase enzymes, used in the degradation of amino acid phenylalanine and in the biosynthesis of the neurotransmitters serotonin (5-hydroxytryptamine, 5-HT), melatonin, dopamine, norepinephrine (noradrenaline), epinephrine (adrenaline), and is a cofactor for the production of nitric oxide (NO) by the nitric oxide synthases. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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