TBCB Antibody (OAGA00140)

Catalog No: OAGA00140

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
12% SDS PAGE
OAGA00140 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
OAGA00140 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using CKAP1(OAGA00140) antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed A549, using CKAP1(OAGA00140) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3
  Cellular Localization: Cytoplasm, cytoskeleton
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 243 of CKAP1 (Uniprot ID#Q99426)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.43 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TBCB
• Gene Full Name: Tubulin-folding cofactor B
• Alias Symbols: CG22, CKAP1, CKAPI
• NCBI Gene Id: 1155
• Protein Name: Tubulin-folding cofactor B
• Uniprot ID: Q99426
• Protein Accession #: NP_001272.2
• Molecular Weight: 27 kDa