STREPTAVIDIN:RPE Accessory Reagent (OOSA09693)

Catalog No: OOSA09693 (Formerly GWB-Q00336)

Size:1ML

Product Info

• Product Format: Lyophilized. Phosphate buffered saline
• Conjugation: R-PE
• Application: Flow cytometry
• Additional Information: Buffer solution: Phosphate buffered saline pH7.2
  Label Protein Molar Ratio: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
  This product conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.
  Preservative: 0.09% - Sodium Azide
  1% - Bovine Serum Albumin
• Reconstitution and Storage: 2°C to 8°C
• Application Info: Flow Cytometry:    Neat
  
• Reference: 1. Van der Nieuwenhof, I. et al. (2001) Differential galactosylation of neuronal and haematopoietic signal regulatory protein-alpha determines its cellular binding - specificity. J. Cell. Sci. 114 (7): 1321 - 1329
  2. Tasker, L. et al. (1997) Immature B cells from neonatal mice show a selective inability to up-regulate MHC class II expression in response to antigen receptor ligation. Int. Immunol. 9: 475 - 484.
  
• Purification: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel. STAR4A conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.

Target Info

• Description of Target: STREPTAVIDIN:RPE
  
• Protein Size (# AA): 159