Stat4 Polyclonal Antibody (OABB00644)

Catalog No: OABB00644 (Formerly GWB-BBP567)

Size:100UG

Lane1: Rat Testis Tissue Lysate
Lane2: U87 Cell Lysate
Lane3:Hela cell Lysate
Lane4:MCF-7 cell Lysate
Lan5: M231 cell Lysate
Lane6:Colo320 cell Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Chicken|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: STAT4 is a human gene. The protein encoded by this gene is a member of the STAT protein family of transcription factors. STAT4 is mapped to chromosome 2q32.2-q32.3 by fluorescence in situ hybridization. STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. Transactivation, electrophoretic mobility shift, and RNA interference analyses showed that Ikaros bound to the STAT4 promoter and was involved in regulation of STAT4 in human T cells.
  Background: STAT4 is a human gene. The protein encoded by this gene is a member of the STAT protein family of transcription factors. STAT4 is mapped to chromosome 2q32.2-q32.3 by fluorescence in situ hybridization. STAT4 is phosphorylated in response to interleukin-12 and is essential for IL12 signal transduction. Transactivation, electrophoretic mobility shift, and RNA interference analyses showed that Ikaros bound to the STAT4 promoter and was involved in regulation of STAT4 in human T cells.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
• Reference: Frucht, D. M., Aringer, M., Galon, J., Danning, C., Brown, M., Fan, S., Centola, M., Wu, C.-Y., Yamada, N., Gabalaway, H. E., O'Shea, J. J.Stat4 is expressed in activated peripheral blood monocytes, dendritic cells, and macrophages at sites of Th1-mediated inflammation.J. Immun. 164: 4659-4664, 2000.
  2. Pang, Y. H., Zheng, C. Q., Yang, X. Z., Zhang, W. J.Increased expression and activation of IL-12-induced Stat4 signaling in the mucosa of ulcerative colitis patients.Cell. Immunol. 248: 115-120, 2007.
  3. Yap, W.-H., Yeoh, E., Tay, A., Brenner, S., Venkatesh, B.STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells.FEBS Lett. 579: 4470-4478, 2005.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 4(STAT4) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: STAT4
• Gene Full Name: signal transducer and activator of transcription 4
• Alias Symbols: signal transducer and activator of transcription 4;signal transducer and activator of transcription 4 variant 3;SLEB11.
• NCBI Gene Id: 6775
• Protein Name: Signal transducer and activator of transcription 4
• Description of Target: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
• Uniprot ID: Q14765
• Molecular Weight: 85941 MW