STAT1 Antibody (OAAB17746)

Catalog No: OAAB17746

Size:400ul

STAT1 Antibody (Cat. #OAAB17746) western blot analysis in CEM, Hela cell line lysates (35ug/lane).
This demonstrates the STAT1 antibody detected the STAT1 protein (arrow).
Dilution: 1:1000

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Purified His-tagged STAT1 protein was used to produced this monoclonal antibody.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Application Info: WB: 1:1000
• Reference: Kristensen I., et al. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
  Schindler C., et al. Proc. Natl. Acad. Sci. U.S.A. 89:7836-7839(1992).
  Ota T., et al. Nat. Genet. 36:40-45(2004).
  Bechtel S., et al. BMC Genomics 8:399-399(2007).
  Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

Target Info

• Gene Symbol: STAT1
• Alias Symbols: CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
• NCBI Gene Id: 6772
• Description of Target: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine-and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
• Uniprot ID: P42224
• Protein Accession #: NP_009330.1, NP_644671.1
• Molecular Weight: 87 kDa