SP3 Antibody (OAGA07495)

Catalog No: OAGA07495

Size:100 ul

SP3 antibody detects SP3 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with SP3 antibody diluted by 1:1000

SP3 antibody detects SP3 protein by western blot analysis.
A. 30 ug Jurkat whole cell extract
B. 30 ug Jurkat nuclear extract
7.5 % SDS-PAGE
SP3 antibody dilution: 1:1000

SP3 antibody detects SP3 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP3 antibody dilution: 1:1000.

SP3 antibody detects SP3 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SP3 antibody dilution: 1:1000.

SP3 antibody detects SP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: SP3 protein stained by SP3 antibody diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunoprecipitation of SP3 protein from Jurkat nuclear extracts using 5 ug of SP3 antibody .
Western blot analysis was performed using SP3 antibody .
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SP3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: SP3
• Gene Full Name: Sp3 transcription factor
• Alias Symbols: SPR2
• NCBI Gene Id: 6670
• Protein Name: transcription factor Sp3
• Description of Target: This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
• Uniprot ID: Q02447
• Protein Accession #: NP_001017371.3
• Nucleotide Accession #: NM_001017371.4